Shake well before use. Turn off UV/ozone. Use 5 mL for each 40 L (10 US gallons). Then use a maintenance dose of 5 mL for each 80 L (20 US gallons) with additions of livestock or the disruption of the biofilter through cleaning or medications.
Antibiotics should be removed with fresh carbon and a water change before adding bacteria. Allow 2 or 3 days for the bacteria to become established before using UV sterilizers or ozone. Recurring problems with high nutrients can indicate overstocking, overfeeding or inadequate filtration.
Product shelf life is 4 years from date of manufacture, see date on package.